Anthrone Test: A Quantitative analysis of Carbohydrates

Anthrone is a tricyclic sweet-smelling ketone. It is utilized for a well-known cellulose measure and in the colorimetric determination of carbohydrates. The anthrones are utilized as a part of drug store as diuretic. They improve the movement of the colon and are in charge of less water reabsorption. They may just be utilized for a short measure of time. The long time use of these elements may cause loss of electrolytes.

Anthrone Test for Carbohydrates:

Carbohydrates are the essential part of the storage and basic materials in the plants. The starches are put away as free sugars and polysaccharides. The essential units of sugars are Monosaccharide. When you want to hydrolyze the starches, it gives monosaccharide, but sill when hydrolyzed, it cannot be part into easier sugars. The hydrolyzed result of Polysaccharide is evaluated by the resultant monosaccharide.

Anthrone Test Principle:

Starches are dried out with concentrated H2SO4 to frame “Furfural”, which gathers with anthrone to shape a green shading complex which can be measured by utilizing colorimetrically at 620nm (or) by utilizing a red channel. Anthrone reacts with dextrins, monosaccharide, disaccharides, polysaccharides, starch, gums and glycosides. If this happens, the yield of shading is where is to frame sugar to starch.

Here is the step by step reactions that occur in this experiment:

Anthrone Test reagents:

To perform any medical or chemical tests, you will need some reagents that are essential and without them, a test cannot be performed. Following are few steps about the recognition and the preparation of a reagent for this specific medical test.

To prepare the reagent you will need to dissolve 200mg of anthrone reagent in concentrated Sulphuric acid which should 100 ml in quantity.

Anthrone Test preparation Procedure:

Unlike previous tests I have written about on my site, this test requires a rather complex process or in fact a long process which is not very difficult. All you need to do is follow each and every step as written in the test and you will be done within few minutes.

In the first step, you will need to measure/weigh the weight of the sample by taking a boiling tube. Make sure that the quantity of sample should be 100 mg.
Keeping it in boiling water bath for three to four hours will hydrolyze the solution. This requires 5 ml of N Hydrochloric Acid. After this whole operating, let the solution cool down till it reaches the room temperature.
Then your next step should be to neutralize the solution. You will need to do this with the help of solid sodium carbonate and let it until the ceasing of effervescence.
Now you will need to make up a volume of 100 and also the centrifuge.
Now take aliquots which should be 0.5 and 1 ml in quantity and then after that collect down the supernatant.
Next step should be to prepare the standards. Here is a sequence in which you should prepare your sequence like 0.2, 0.4, 0.6, 0.8 and then at the end 1 ml.
Volumes in all the test tubes should be 1 ml. Now this is what you will do. Equalize the volume in all test tube to 1 ml and this can be done by using distilled water. Once being done with that, you will need to add 4 ml of anthrone reagent which I have already told you how to prepare.
Now take a boiling water bath and heat it carefully for 8 consecutive minutes. After the heating is done move forward to next step.
Now rapidly cool down the solution and carefully read the color till it changes from green to dark green color. This should be done at 630 nm.
Your experiment is done. Now plot some graphs to study the result.

Anthrone Test Results:

Graphs should be plotted like standard concentrations on the x-axis and on y-axis should be absorbance of the material. Being done with all the standard experiment procedure, it is time for us to move on to the calculations.

Calculations of the Anthrone Test:

The amount of carbohydrates that are present in 100 mg of the solution sample = (mg of glucose / volume of the test sample) x 100. This is the formula for final result calculation.

Precautions to perform Anthrone Test:

There is always a list of precautions that are required to be taken before and while performing any experiment.

Make all the measurements very carefully. All the solutions and samples should be of exact amount as mentioned in the experiment.
While heating the solution the boiling water bath, do it very carefully. Also bring down the temperature rapidly to room temperature. Taking care of the temperature is important because it readily affects the results.
Take care of the time that you are giving to heat the sample as well as to the accurate temperature.

These precautions, once being taken care of, should ensure a 100% positive test.

Final Words:

So this was Anthrone test to find a number of carbohydrates in a solution. These guides are made for your help and if you feel that there is something lacking in the experiment such as any kind of info that was required for the test to be performed, you can let us know simply by commenting in the comments section. You can also leave your feedback on how well did this guide help you. Keep visiting our site for more medical experiments in the future.