Today we are going to do the determination of Plasma Protein by Biuret Method. Before we move on to the test, we will need to tell you about Biuret method. The presence of peptide bonds and other things such as Plasma Proteins can be checked with the help of this easy and most successful chemical test named Biuret Method. The presence of proteins can be found with the help of this test and this is the main use of this test. Today, we are going to determine the presence of Plasma Proteins by Biuret Method. Also, in this article, I am going to give you the complete procedure of this test, its principal and also the reagent being used in the test. This will be one of the easiest guides you have read on the internet to learn about the biuret method of finding plasma proteins.
Principal and Reagents Of Biuret Method:
The principal of this test is quite a simple one. Once the test is performed, we can determine the presence of proteins by the changing color of the solution. There are different compounds and solutions taking part in the test including the biuret reagent which we are going to prepare ahead.
The elements or compounds that are used to prepare the biuret reagent include sodium hydroxide, hydrated copper with potassium sodium tartrate. The reason for adding the 3rd compound mentioned is the stability of the cupric ions in the reagent. Once these compounds are added in a fixed proportion, the biuret reagent is prepared for the test. Make sure that you take important precautions while preparing the reagent and also while performing the rest of the test. Once you have all the things including the reagent, we can proceed to perform the biuret method.
The procedure of Determination of Plasma Protein by Biuret Method:
The procedure for determination of plasma proteins by biuret method is a very simple one. It takes only few steps to do the procedure and then we can get the desired results. Let me mention again that you must take care important precaution written in the precautions section. Once you have taken care of them and have all the things including the reagent, you can follow the following step by step guide to perform this test.
- In the first step, a base is taken which could be either potassium or potassium hydroxide and its amount must be 1%.
- Next step is to treat this base with a water sample. Also, a few drops of copper sulfate must be added during the procedure.
- In the step three, you start getting results just after performing the above two steps.
Determination of Plasma Protein by Biuret Method Results:
After performing the first two steps in the test, if the color of the solution turns to purple, it means that the proteins are present in the solution. If the purple color doesn’t appear, we are sorry to say that your test is negative. The purple color is the major indicator for the presence of proteins and this gives an easy indication really.
Before performing any scientific test in the lab, make sure that you take proper precautions. These precautions ensures the best results and your safety as well. Written below are the precautions for this test.
- Make sure that you have measured amount of all the chemicals correctly.
- While making the reagent, taking notice of the proportions is must.
- Do not taste any of the chemicals because that could cause serious problems.
Once you have taken care of these things, your test will be a happy one. So, was this article helpful for you? Don’t forget to leave us your feedback about the test in the comments section. You can also leave us any queries in the section below. Keep visiting our website All Med Tests for more guides on scientific tests.